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Wavelet-based synchronization of nongated confocal microscopy data for 4D imaging of the embryonic heart

M. Liebling, A. S. Forouhar, M. Gharib, S. E. Fraser, and M. E. Dickinson

in Wavelets XI, Proceedings of SPIE, vol. 5914, eid 591409, 6 pages, 2005.
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Abstract With the availability of new confocal laser scanning microscopes, fast biological processes, such as the blood flow in living organisms at early stages of the embryonic development, can be observed with unprecedented time resolution. When the object under study has a periodic motion, e.g. a beating embryonic heart, the imaging capabilities can be extended to retrieve 4D data. We acquire nongated slice-sequences at increasing depth and retrospectively synchronize them to build dynamic 3D volumes. Here, we present a synchronization procedure based on the temporal correlation of wavelet features. The method is designed to handle large data sets and to minimize the influence of artifacts that are frequent in fluorescence imaging techniques such as bleaching, nonuniform contrast, and photon-related noise. © 2005 Society of Photo-Optical Instrumentation Engineers. DOI 10.1117/12.615232

@Conference{ liebling.forouhar.ea:05a,
  author = {Michael Liebling and Arian S. Forouhar and Morteza Gharib
    and Scott E. Fraser and Mary E. Dickinson},
  editor = {Manos Papadakis and Andrew F. Laine and Michael A. Unser},
  collaboration = {},
  title = {Wavelet-based synchronization of nongated confocal
    microscopy data for 4D imaging of the embryonic heart},
  publisher = {SPIE},
  year = {2005},
  journal = {Wavelets XI},
  volume = {5914},
  number = {1},
  eid = {591409},
  numpages = {6},
  pages = {591409},
  location = {San Diego, CA, USA},
  url = {http://link.aip.org/link/?PSI/5914/591409/1}
}

Copyright © 2005 Society of Photo-Optical Instrumentation Engineers. This paper was published in the above mentioned proceedings and is made available as an electronic reprint with permission of SPIE. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.