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Mutual registration allows for synchronization of bright-field and fluorescence microscopy images of the beating embryonic zebrafish heart. Left panel: brightfield microscopy sequence. Right panel: sequence obtained with widefield
epi-fluorescence microscopy. Sequences synchronized with a mutual-information-based method.

M. Liebling, H. Ranganathan, "Wavelet Domain Mutual Information Synchronization of Multi-Modal Cardiac Microscopy Image Sequences," invited paper, Proceedings of the SPIE Conference Optics & Photonics, Wavelets XIII, San Diego CA, USA, August 2-4, 2009, vol. 7446, 744602, 6 pages, doi: 10.1117/12.826519.