Imaging fast moving objects requires high brightness and signal to noise ratio, while maintaining high frame-rate to minimize the effects of motion blur. In this left ventrolateral view of a 7 days post fertilization old transgenic Tg(cmlc2:eGFP) larval zebrafish the beating embryonic heart is imaged at 2, 10, 30 and 60 frames per second (that is 500 ms, 100 ms, 30 ms and 15 ms integration time, respectively) revealing the need for high framerates to capture morphology because of the imaged structures' high velocity.
J. Ohn, H.-J. Tsai, M. Liebling, "Joint Dynamic Imaging of Morphogenesis and Function in the Developing Heart," Organogenesis, vol. 5, iss. 4, Oct./Nov./Dec. 2009.
