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Brightfield high-speed imaging and flow analysis allows imaging at high framerates and resolve motion of individual blood cells in the cardiac tract for subsequent flow velocity estimation.
Left Panel: Brightfield image of the beating embryonic heart of a wild type 82hpf zebrafish heart at 3000 frames per second. Center Panel: Qualitative motion representation by superposition of 5 successive frames, each with a hue corresponding to its relative time stamp. Right Panel: Local motion estimation of red blood cells and heart wall motion reveals velocities as high as 20 mm/s. Scale bar is 20μm.

J. Ohn, H.-J. Tsai, M. Liebling "Joint Dynamic Imaging of Morphogenesis and Function in the Developing Heart," Organogenesis, vol. 5, iss. 4, 2009.